covalab ACCELERATE YOUR COVID-19 RESEARCH WITH OUR PRODUCTS CATALOG

 

covid19

 

Variants          Recombinant Proteins          Antibodies           Peptides

 

sars-cov-2


SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019 nCoV (2019 Novel Coronavirus) represents a pandemic threat that has been declared a public health emergency of international concern by WHO, mobilizing the actors of all the fields to find new means to fight against this virus, in particular in the field of research.

Covalab is making all the effort to help you in your research by offering wide range of tools such as specific proteins from SARS-Cov-2, antibodies or immunoassays involved in the metabolic pathways of infection, to accelerate the research, diagnostic and treatment of COVID-19.

2019 nCoV is an enveloped, single, and positive-stranded RNA virus. The virus encodes four major structural proteins that have played important roles in causing the infection (*): Spike protein (S protein), nucleocapsid protein (N protein), membrane protein (M protein), and small envelope protein (E protein) (Fig.1).

*: Fehr, A.R. & Perlman, S. 2015. Coronaviruses: an overview of their replication and pathogenesis. Methods Mol Biol 1282, 1-23

 

S protein is a large type I transmembrane protein, composed of two sub-units S1 and S2. S protein is essential for both host specificity and viral infectivity.

For viral entry, the S1 sub-unit binds via its RBD (Receptor Binding Domain) to the cellular receptor angiotensin-converting enzyme 2 (ACE2) and after cleavage by proteases (Furin and TMPRSS2), the active S2 sub-unit fuses to the viral membranes with the host cell membrane. The spike protein is reported to induce the neutralizing-antibody and T-cell responses, as well as protective immunity. The CoV spike (S) glycoprotein therefore represent the key target for vaccines, therapeutic antibodies, and diagnostics (*).

*: Hoffmann, M. et al. 2020. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell

 

SARS-Cov-2 can be triggered to fuse at either the plasma membrane or the endosomal membrane, and the route of entry is dependent on protease availability (Fig. 2).

sars viral cycle

 

The virus antigens are presented to the immune-system (APCs, macrophage, …) which in turn produce a range of pro- inflammatory "cytokines” (e.g. IL6, IFNα, IFNβ, IFNγ, IFNλ…) and TNF-α (*). In some cases, these cytokines proliferate and activate an uncontrolled and devastating pro-inflammatory response in the host.

* : Guo, Y.R. et al. 2020. The origin, transmission and clinical therapies on coronavirus disease 2019 (COVID-19) outbreak - an update on the status. Mil Med Res 7, 11

tnf pathway

 

 

Coronavirus Research Products:

For all information about these following products, contact us at contact@covalab.com


 

bubble SARS-CoV-2 Variants

 

RBD variants
 Description Packaging #Catalog Price

Indian Delta Plus variant – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations K417N-L452R-T478K, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190016 449€

Indian Delta variant (B.1.617.2) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations L452R-T478K, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190015 449€

Indian Kappa variant (B.1.617) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations L452R-E484Q, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190013 449€

UK variant (501Y.V1) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations N501Y, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190012 449€

UK V2 variant (484-501) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations E484K-N501Y, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190010 449€

South Africa variant (501Y.V2) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations K417N-E484K-N501Y, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190009 449€

Brazil variant (484K.V2) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations K417T-E484K-N501Y, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190011 449€

California variant (452R.V1) – HEK

SARS CoV-2 recombinant Spike RBD protein, mutations L452R, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190014 449€

 

N variants
 Description Packaging #Catalog Price

UK variant (B.1.1.7) – HEK

SARS CoV-2 recombinant Nucleoprotein protein, mutations D3L-R203K-G204R-S235F, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190006 759€

UK variant (DL3-S235F) – HEK

SARS CoV-2 recombinant Nucleoprotein protein, mutations D3L-S235F, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190005 759€


 

bubble Recombinant Proteins

 

Recombinant Spike Protein
Description Packaging #Catalog Price

Soluble Spike - HEK / E.coli

Recombinant soluble Spike (S) protein (ECD), amino acids 13-1213 C-terminal hexa-His-Tag (HEK cells) or N-terminal GST, C-terminal hexa-His-Tag (E.Coli).

CURRENTLY UNDER VALIDATION

100 µg Contact us

S1-Subunit – HEK

SARS-CoV-2 recombinant Spike (S) protein S1-Subunit, amino acids 13-685, C-terminal hexa-His-Tag (HEK cells).

 100 µg CovID190002 380€

Variant S1-Subunit (K417N-E484K-N501Y - D614G)

Variant SARS-CoV-2 recombinant Spike (S) protein S1-Subunit, mutation K417N-E484K-N501Y - D614G, C-terminal hexa-His-Tag (HEK cells)

100 µg Contact us

RBD – HEK

SARS-CoV-2 recombinant Spike (S) protein (receptor-binding domain, RBD), amino acids 319-541, C-terminal hexa-His-Tag (HEK cells).

 100 µg CovID190003  380€
1 mg CovID190003 Contact us

S2-Subunit – HEK

Recombinant Spike (S) protein (S2 ECD), amino acids 686-1213, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190008 637€

 

WB spike sds page spike elisa spike

WB: Recombinant Virus SARS-CoV-2 Spike protein S1 subunit - HEK293 - his tag (lane 1, 0.5 µg, ref: CovID190002), Spike protein (RBD) - HEK293 - His tag (lane 2, 0.5 µg, ref: CovID190003), and RBD competitor (lane 3, 0.5 µg), showing a glycosylated band at 130 kDa and 34 kDa respectively.

Antibodies dilution: Primary SARS‑CoV-2 Spike - S1 subunit Antibody (27H7) (1000e, ref: mab0163-P) and secondary anti-anti-His Tag (HIS.H8 / EH158) (1mg/ml, ref: mab90001-P).

SDS-PAGE: Recombinant Virus SARS-CoV-2 Spike protein S1 subunit - HEK293 - his tag (lane 1, 2µg, ref: CovID190002), Spike protein (RBD) - HEK293 - His tag (lane 2, 2µg, ref: CovID190003), and RBD competitor (lane 3, 2µg), analyzed by SDS-PAGE (7,5% gel) with Coomassie Blue, showing a glycosylated band at 130 kDa and 34 kDa respectively.

ELISA: Immunoreactivity validation by ELISA test using coated Spike protein S1 subunit  – HEK – his tag (ref: CovID190002) and Spike protein (RBD) – HEK – His tag (ref: CovID190003).

Antibodies dilution: Primary SARS‑CoV‑2 Spike-RBD domain antibody (ref: pab01035-P) and secondary anti-rabbit HRP (2000e, ref: lab0252).

Recombinant N protein   
 Description Packaging #Catalog Price

Nucleoprotein (N) – HEK

SARS-CoV-2 recombinant N protein, amino acids 1-419, C-terminal hexa-His-Tag (HEK cells).

100 µg CovID190001 449€

Nucleoprotein (N) – E.coli

SARS-CoV-2 recombinant N protein, amino acids 1-419, C-terminal hexa-His-Tag (E.Coli).

100 µg CovID190007 449€

 

 

Recombinant E protein
 Description  Packaging #Catalog Price 

Protein E - HEK / E.coli

SARS CoV-2 recombinant E protein, amino acids 1-75, C-terminal hexa-His-Tag (HEK cells) or N-terminal GST, C-terminal hexa-His-Tag (E.Coli). 

CURRENTLY UNDER VALIDATION

 100 µg Contact us 

 

 

Recombinant M protein    
Description  Packaging #Catalog Price 

Membrane protein (M) – HEK / E.Coli

SARS CoV-2 recombinant M protein, amino acids 1-222, C-terminal hexa-His-Tag (HEK cells) or N-terminal GST, C-terminal hexa-His-Tag (E.Coli).

CURRENTLY UNDER VALIDATION

100 µg  Contact us 

 

 

Recombinant ACE-2 protein    
Description Packaging  #Catalog Price 

 ACE2 - HEK

Recombinant ACE2 protein (ACE2), amino acids 19-7615, C-terminal hexa-His-Tag (HEK cells).

100 µg pro30005 449€ 

 

 


 

bubble Antibodies

 

Spike (S) Protein
Description   Host Species   #Catalog   Price
Monoclonal SARS‑CoV-2 Spike - S1 subunit Antibody (27H7)   Mouse   mab0163-P   480€
Monoclonal SARS‑CoV-2 Spike - RBD domain Antibody Mouse mab0164-P 480€
Monoclonal SARS‑CoV-2 Spike - S2 subunit Antibody Mouse mab0168-P 480€
Polyclonal SARS‑CoV S1 - RBD domain Antibody   Human   pab01035-P   449€
 
Nucleocapsid (N) Protein
Description   Host Species   #Catalog   Price
Monoclonal SARS-CoV-2 Nucleoprotein Antibody Mouse   mab0169-P   480€
Polyclonal SARS‑CoV‑2 Nucleoprotein Antibody Currently under validation Contact us

 

 

Matrix (M) Protein
Description   Host Species   #Catalog   Price
Polyclonal SARS‑CoV‑2 Matrix protein Antibody   Currently under validation    Contact us
 
Envelope (E) Protein
Description   Host Species   #Catalog   Price
Polyclonal SARS‑CoV‑2 Envelope protein Antibody     Currently under validation   Contact us

 

 

ACE2
Description   Species   #Catalog   Price
ACE2 (aa18-237) antibody (1G4) Human   mab72035    430€
Angiotensin Converting Enzyme 2 (s754) (ACE2) Human, Ms, Rat   mab60668    449€
Angiotensin Converting Enzyme 2 (ACE2) Human, Ms, Rat   pab60452    449€
Angiotensin Converting Enzyme 2 (ACE2) Human, Ms, Rat   pab60453    449€
Angiotensin Converting Enzyme 2 (ACE2) Human, Ms, Rat   pab60454    449€
Angiotensin Converting Enzyme 2 (ACE2) Human, Ms, Rat   pab60455    449€
 

  

 


 

bubble Peptides

 

peptide library

 

Thanks to our expertise in the fields of peptide design, synthesis and modification, we provide a high-quality peptide synthesis service, including a wide range of chemical modifications.

In addition, we offer a specific peptide library for research on SARS-CoV-2 form crude, >70% to very hight purity (>98%).

 

Protein name Lenght Uniprot ID
SARS-CoV-2 Spike Glycoprotein 1 273 aa P0DTC2 
HCoV-229E Spike Glycoprotein 1 173 aa P15423
SARS-CoV-2 N 419 aa P0DTC9
SARS-CoV-2 E 75 aa P0DTC4
SARS-CoV-2 M 222 aa P0DTC5
SARS-CoV-2 ORF3a 275 aa P0DTC3
and many others...

 

Contact us for design and more information about peptide synthesis at enquiries@covalab.com.

All our team are available for all requests.