Anticorps RECOMBINANT PROTEIN

 

Covalab provides a large and various bio production offer for proteins (tag, ...). We are using eucaryotic or prokariotic systems and offering a large range of bioproduction scale starting from mililiter and up to 12 L bioreactor. All proteins production occurs in our ISO-certified manufacturing facility.
Reactivity, high quality production and tailor made services is our main objective. Thanks to nearly 30 years of experience and our highly trained scientific team we can adapt our process to answer all your specific needs and meet your goal.

 

Eukaryote

Eukaryote (CHO, HEK)

Prokaryote

Prokaryote (E. coli)

Transfection

Transient & stable

transfection

Large scale application

Large scale application

(from mg to g)

Tailor made service prot

Tailor-made service

Fee service

Fee for service

Animal free

Animal free

Quality control

Quality control

 

 

Bulle Eukaryotic production (CHO, HEK)

 

  • Bibliography study
  • Sequence analysis
  • Expression and purification strategy proposal (tag selection)
  • GO / NO GO step
  • Primers design
  • Gene synthesis including a tag (codon optimization)
  • Subcloning into an expression vector for eukaryotic model
  • Amplification and purification of the plasmid
  • Control by sequencing
  • GO / NO GO step
  • Transient transfection in CHO / HEK cells
  • Pilot production small scale volume (≈ 100 mL)
  • Protein purification by affinity chromatography
  • Protein characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation
  • Material transfert for validation
  • GO / NO GO step
  • Transient transfection / Stable transfection in CHO / HEK cells
  • In vitro production (1 - 12 L)
  • Protein purification by affinity chromatography
  • Polishing step (SEC, ion exchange...)
  • Protein characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation (BCA...)
  • Endotoxin level, HCPs
  • Production, purification and quality control report

 

  

Total project duration: From 11 weeks (≈ 3 months)

  

Project duration

   

   

Eukaryotic production - Recombinant protein

 

 


 

Bulle Prokaryotic production (E. coli)

 

  • Bibliography study
  • Sequence analysis
  • Expression and purification strategy proposal (tag selection...)
  • GO / NO GO step
  • Primers design
  • Gene synthesis including a tag (codon optimization)
  • Subcloning into an expression vector for prokaryotic model
  • Amplification and purification of the plasmid
  • Control by sequencing
  • GO / NO GO step
  • Bacterial culture (E. coli) in tubes of 3 mL and best conditions selection
  • Pilot production small scale volume (≈ 200 mL)
  • Protein purification by affinity chromatography
  • Protein characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation
  • Material transfert for validation
  • GO / NO GO step
  • Production of 10 mg (for a yield of 0.5 mg /L, up to 5 L)
  • Purification by affinity chromatography (in the case of tag)
  • Protein purification by affinity chromatography
  • Protein characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation (BCA...)
  • Production, purification and quality control report

 

 

Total project duration: From 11 weeks (≈ 3 months)

  

Project duration

 

 

 

 


 

Bulle Additional services

 

Validation & Quality control

Please click here to visit our page about validation and QC and get more information.

 

Purification

Please click here to visit our page about purification and get more information.

 

Labelling

Please click here to visit our page about labelling and get more information.