Anticorps RECOMBINANT ANTIBODY

 

Covalab provides bio production services for antibodies (including their fragments). We are using eucaryotic systes and offering a large range of bioproduction scale starting from mililiter and up to 12 L bioreactor. All antibodies production occurs in our ISO-certified manufacturing facility.
Reactivity, high quality production and tailor made services is our main objective. Thanks to nearly 30 years of experience and our highly trained scientific team we can adapt our process to answer all your specific needs and meet your goal.

 

Tailor made service

Tailor-made service

Fee service

Fee for service

Animal free

Animal free

Large scale application

Large scale application

(from mg to g)

 

 

Bulle Eukaryotic production (CHO)

 

  • Bibliography study
  • Sequence analysis
  • Expression and purification strategy proposal (tag selection)
  • GO / NO GO step
  • Primers design
  • Gene synthesis including a tag (codon optimization)
  • Subcloning into an expression vector for eukaryotic model
  • Amplification and purification of the plasmid
  • Control by sequencing
  • GO / NO GO step
  • Transient transfection in CHO cells
  • Pilot production small scale volume (≈ 100 mL)
  • Antibody purification by affinity chromatography
  • Antibody characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation
  • Material transfert for validation
  • GO / NO GO step
  • Transient transfection / Stable transfection in CHO cells
  • In vitro production (1 - 12 L)
  • Antibody purification by affinity chromatography
  • Polishing step (SEC, ion exchange...)
  • Antibody characterization (SDS-Page, ELISA, flow cytometry, HPSEC, WB...) and yield estimation (BCA...)
  • Endotoxin level, HCPs
  • Production, purification and quality control report

  

Total project duration: From 11 weeks (≈ 3 months)

  

Project duration

   

   

Bioreactor_SDS Page

 

 


 

 

Bulle Additional services

 

Prokaryotic production

We can offer procaryotic production on request (VHH, scFv), please contact us.

 

Validation & Quality control

Please click here to visit our page about validation and QC and get more information.

 

Purification

Please click here to visit our page about purification and get more information.

 

Labelling

Please click here to visit our page about labelling and get more information.