His-Tag antibody (HIS.H8 / EH158)

poly-histidine, polyhistidine
Histidine tagged proteins
6 His epitope tag, HHHHHH epitope tag,
Hexa His tag, 6X His tag
As low as €134.40
In stock
Ref
00012927
His-Tag antibody (HIS.H8 / EH158)
More Information
ID Covalab mab90001-P
Product type Primary antibodies, Tags
Clonality Monoclonal antibody
Clone HIS.H8 / EH158
Isotype IgG2b
Raised in Mouse
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Activity cross reaction Reacts with the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Labelling None
Form Purified
Preservative NaN3 0.05%
Storage instructions Short term storage at +4°C (add 0.1% NaN3).
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Target poly-histidine
Applications ELISA, ICC, IP, WB
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
WB 1:1000 - 1:3000
ICC 1:500 - 1:2000
Publication * Ossareh-Nazari B et al. Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy. J Cell Biol. 2014 Mar 17,204(6):909-17.
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