FAQ POLYCLONAL ANTIBODIES
Some frequently asked questions about polyclonal antibodies (click on the question to see the answer):
We can assist you in designing your peptide. We use a combination of prediction software and in-house expertise to select the most relevant immunogenic peptide sequences. We select peptide sequences for immunisation based on a number of criteria:
- antigenicity, hydrophobicity and accessibility,
- sequence alignment (BLAST) to confirm peptide specificity,
- secondary structure analysis (signal peptide, transmembrane domains, DNA binding domain…),
- predictive study to determine potential difficulties with the synthesis.
Phosphorylation is one of the major post-translational modifications. Visit the section of our website dedicated to post-translational modification as immunogens for more specific information. Briefly, the phosphorylated peptide is first conjugated to a carrier protein and used to immunise the host animals. Following the final bleed phospho-specific antibodies will be purified by a 3-step affinity purification procedure:
- the immune serum is loaded onto a column with the control peptide coupled to agarose beads, thus retaining unmodified peptide-specific antibodies,
- the flow-through is then loaded onto a column with the modified peptide coupled to agarose beads, thus retaining the modified peptide-specific antibodies,
- after elution, the eluate is assayed by ELISA against both peptides to control its immunoreactivity and its specificity against the modification.