FAQ about polyclonal antibodies

Some frequently asked questions about polyclonal antibodies (click on the question to see the answer):

  • What animal is used to raise polyclonal antibodies?
  • How much antigen is required for the project?
  • How can I supply my protein?
  • How long is your standard protocol?
  • How many injections and bleeds are performed in the standard protocol?
  • Do you help with the peptide design?
  • Do you raise phosphospecific antibodies?
  • Do you provide antibody labelling?
  • Do I need sodium azide in my serum?
  • How much serum will I receive?
  • Why are my test bleeds red?
  • How do I store the serum?
  • How do I interpret my ELISA results?
  • What does antibody titre mean?
  • When do I know whether an immune response to a specific antigen has occured?
  • Do you guarantee a minimum titre?
  • The immune response against the peptide/protein at the end of the protocol is low. Will an extension of the project improve the titre?
  • When is an extension of the immunisation protocol advisable?
  • How long can a project be extended?
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