Inhibitors of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases has been identified and designated Smac/DIABLO. Smac is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. The N-terminal amino acids of Smac are required for binding to IAPs and for activation of caspases. Smac is expressed in a variety of human and mouse tissues.
|Product type||Primary antibodies|
|Immunogen||Synthetic peptide corresponding to the C-terminus part of (aa 225-239) of human Smac (accession no. AAF87716)|
|Activity cross reaction||Reacts with human, mouse and rat Smac.|
|Species||Hu, Ms, Rat|
|Storage instructions||Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.|
|Working dilutions||Optimal dilution should be determined by the end user.
The following are guidelines only:
WB (1 µg/ml)
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