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DNMT1 (4G11-C7) antibody
DNA (cytosine-5)-methyltransferase 1
CXXC-type zinc finger protein 9, CXXC9
DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI
Gene name : DNMT1
CXXC-type zinc finger protein 9, CXXC9
DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI
Gene name : DNMT1
As low as
$537.90
In stock
Ref
00096628
Methylation of DNA at cytosine residues is important in regulation of gene expression and genomic imprinting. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked to development of cancer. Thus far three families of mammalian DNA methyltransferase genes have been identified : DNMT1, DNMT2, and DNMT3.
DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.
DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.
| ID Covalab | mab60115 |
|---|---|
| Product type | Primary antibodies |
| Clonality | Monoclonal antibody |
| Clone | 4G11-C7 |
| Isotype | IgG2b |
| Raised in | Mouse |
| Immunogen | Synthetic peptide corresponding to aa 637-650 of human DNMT1 (accession no. NP_001370). |
| Activity cross reaction | Reacts with human, mouse and zebrafish DNMT1. |
| Species | Hu, Ms, Zfi |
| Labelling | None |
| Form | Purified (protein G) |
| Preservative | 0,09% NaN3 |
| Storage instructions | This antibody is stable for at least one year at -20°C. Avoid repeated freeze-thaw cycles. |
| Target | DNMT1 |
| Uniprot ID | P26358 |
| Gene ID | 1786 |
| Applications | ELISA, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : WB (2 µg/ml) |
| Positive control | ES and H1299 cell lysates. |
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