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DMPO Nitrone Adduct (N1664A) antibody
55 dimethyl 1 pyrroline N oxide nitrone adduct
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$685.30
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00096622
Free radicals are highly reactive and generally short-lived species. Hence, most studies of free radicals have been restricted to analysis of end products formed following their interaction with cellular biomolecules. One technology that has permitted direct study of free radicals is electron spin resonance (ESR). For very short lived radicals, spin traps have been employed to generate longer lived radicals that are more amenable to ESR analysis.
The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable.
The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable.
| ID Covalab | mab60109 |
|---|---|
| Product type | Primary antibodies |
| Clonality | Monoclonal antibody |
| Clone | N1664A |
| Isotype | IgG1 |
| Raised in | Mouse |
| Immunogen | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin. |
| Activity cross reaction | Reacts with DMPO, DMPO octanoic acid, DMPO-protein adducts, and DMPO-DNA adducts DMPO Nitrone Adduct. It does not cross react with non-adducted proteins or DNA. |
| Species | All Sp. |
| Labelling | None |
| Form | Purified (protein G) |
| Preservative | NaN3 0.09% |
| Storage instructions | This antibody is stable for at least one year at -20°C. |
| Target | DMPO Nitrone Adduct |
| Applications | ELISA, ICC, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : ELISA (1 - 10 µg/ml) ICC (10 µg/ml) WB (1 - 10 µg/ml) |
| Positive control | DMPO nitrone adducts of metmyoglobin. |
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