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CaMKII (6G9) antibody
CamK2A, CamK2B, CamK2, CamK2D, CAMKA, CamK2G
As low as
$660.00
In stock
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00096569
CaMKII, a member of the calcium /calmodulin-activated protein kinase family, functions in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron.
CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.
CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.
| ID Covalab | mab60056 |
|---|---|
| Product type | Primary antibodies |
| Clonality | Monoclonal antibody |
| Clone | 6G9 |
| Isotype | IgG1 |
| Raised in | Mouse |
| Immunogen | Partially purified rat CaMKII |
| Activity cross reaction | Reacts with phosphorylated and non-phosphorylated forms of mouse CaMKII. Cross-reacts with rat and bovine protein. |
| Species | Bov, Ms, Rat |
| Labelling | None |
| Form | Purified (protein G) |
| Preservative | Nan3 0.09% |
| Storage instructions | Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles. |
| Target | CaMKII |
| Applications | ICC, IF, IHC, IP, RIA, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only: IHC (10 µg/ml) RIA (3 - 5 µg/ml) WB (0,1 - 1 µg/ml) A band of ~50-60 kDa is detected (predicted molecular weight: 56 kDa). |
| Positive control | Rat brain tissue extract |
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