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Bacterial Transglutaminase (mTG)
Protein-glutamine-γ-glutamyltransferase, Protein-glutamine gamma-glutamyltransferase
microbial transglutaminase, BTG
Transglutaminase, TGase
Gene name: MTG
microbial transglutaminase, BTG
Transglutaminase, TGase
Gene name: MTG
As low as
$473.00
In stock
Ref
00012429
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These
enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
| ID Covalab | opr0054 |
|---|---|
| Uniprot ID | P81453 |
| Target | Microbial / Bacterial transglutaminase |
| Preservative | None |
| Storage instructions | Store working aliquots at a minimum of -20°C Avoid repeated freezing and thawing Delivery at ambient temperature is possible |
| Description | Microbial (bacterial) transglutaminase is recombinantly produced in the inactive zymogenic form (Pro-MTG) in E. coli in a shaker flask fermentation. To achieve the production of larger amounts of Pro-MTG, a stirred tank fermentation process was developed. For this purpose, the expression plasmid was changed. Essentially, an expression plasmid carrying a kanamycin-resistance gene is used instead of the former expression plasmid, which contained a β-lactamase-gene. The fermentation medium was changed, however, no animal derived media components are used in the stirred tank fermentation protocol. |
| Raised in | E. coli |
| Species | Str. |
| Form | Recombinant |
| Poids moléculaire | 38 kDa |
| Principe | mTG can be used for labelling, immobilisation, conjugation and modification of proteins. |
| Pureté | > 98% |
| Product type | Proteins |
| Applications | FA |
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