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Nucleolin pT84 antibody
Protein C23
Gene name : NCL
phospho Nucleolin, Nucleolin phospho-Thr 84, NCL-T84p
Gene name : NCL
phospho Nucleolin, Nucleolin phospho-Thr 84, NCL-T84p
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00106849
ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
| ID Covalab | pab0969-P |
|---|---|
| Product type | Primary antibodies |
| Clonality | Polyclonal antibody |
| Raised in | Rabbit |
| Immunogen | Synthetic peptide of human Nucleolin with a phoshothreonine at position 84 |
| Activity cross reaction | Reacts with Nucleolin with phoshothreonine 84 (pT84) from human and mouse. Cross reacts with phoshothreonine 106 (pT106). Do not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of P-NCL can be done during mitosis or in G2 phase, no signal in G1S phase. |
| Species | Hu, Ms |
| Labelling | None |
| Form | Purified (protein A) |
| Preservative | None |
| Storage instructions | Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt. |
| Target | Nucleolin pT84 |
| Uniprot ID | P19338 |
| Gene ID | 4691 |
| Applications | IF, IP, WB |
| Working dilutions | Optimal dilutions should be determined by the end user. The following are guidelines only: - WB : 1/200 to 1/2000 - IF: 1/200 to 1/1000 |
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