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Anti-Mitosin Antibody
CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin
As low as
$734.00
In stock
Ref
00123637
Anti-Mitosin Antibody
| ID Covalab | mab60672 |
|---|---|
| Product type | Primary antibodies |
| Clonality | Monoclonal antibody |
| Clone | 14C10 |
| Isotype | IgG2a |
| Raised in | Mouse |
| Immunogen | GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin. |
| Activity cross reaction | This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation. |
| Species | Human |
| Form | Purified |
| Storage instructions | This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles. |
| Target | Mitosin |
| Uniprot ID | P49454 |
| Gene ID | 1063 |
| Applications | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Working dilutions | Immunoblotting: use at 1-10 ug/mL. Immunoprecipitation: use at 1-10 ug/mL. Immunohistochemistry: use at 10 ug/mL with frozen or paraffin-embedded sections. Positive controls: Human tissue containing rapidly proliferating cells, such as lymphoid germinal centers. |
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