ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
|Product type||Primary antibodies|
|Immunogen||Synthetic peptide of human Nucleolin|
|Activity cross reaction||Reacts with Nucleolin from human, mouse and chicken. Reacts with all the modified and unmodified forms of nucleolin|
|Species||Chick, Hu, Ms|
|Form||Purified (protein A)|
|Storage instructions||Short term storage at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
|Applications||IF, IP, WB|
|Working dilutions||Optimal dilutions should be determined by the end user.
The following are guidelines only:
- WB : 1/500 to 1/2000
- IF: 1/200 to 1/1000
|Publication||* I Ugrinova et al. Nucleolin Interacts and Co-Localizes with Components of Pre-Catalytic Spliceosome Complexes. Sci, 2019
* Cong R et al. Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription. Nucleic Acids Res. 2012 Oct:40(19):9441-54.
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