Beta-actin is commonly used as loading control due to its ubiquitous expression in cells.
|Product type||Loading controls, Primary antibodies|
|Immunogen||beta-Actin N-terminal peptide-KLH conjugates|
|Activity cross reaction||Reacts with 42kDa native and denatured forms of beta-actin from human, mouse, rat, rabbit, and chicken.|
|Species||Chick, Hu, Ms, Rab, Rat|
|Storage instructions||Short term storage at +4°C (add 0.1% NaN3).
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
|Applications||ELISA, FC, ICC, IF, IP, WB|
|Working dilutions||Optimal dilution should be determined by the end user.
The following are guidelines only :
WB (1:1000 - 1:5000)
FC (0.1 - 1µg for 106^ cells)
ICC/IF (10 µg/ml)
IP (5 µg/ml)
|Publication||* Deygas M et al. Nat Commun. 2018 Oct 31;9(1):4545. (WB, Hu)|
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