Human IFNg / IL-5 Dual Fluorospot Set (non-sterile plate)

IFN gamma
As low as $450.00
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00099560
Covalab Dual Fluorospot Sets include capture antibody for cytokine 1, FITC-conjugated detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, capture antibody for cytokine 2, biotinylated detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA. Kit can be sold with or without plates.
More Information
ID Covalab kit30048
Principe After cell stimulation, locally produced cytokines are captured by IFNγ and IL-5 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IFNγ FITC conjugated antibody and a biotinylated anti-IL-5 antibody. Those are in turn recognized by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IFNγ production while IL-5 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.
Applications Fluorospot
Detection method Fluorescent
Activity cross reaction Reacts with both natural and recombinant human IFN-g and IL-5. Cross-reacts with monkey IFN-g. Cross-reactive with simian IFN-g.
Species Hu
Assay type Sandwich ELISA
Assay duration 3h30 after cell stimulation
Form Non-sterile plates
Target IFNg / IL-5
Product type Kits
Sensitivity Optimal dilution shloud be determined by the end user.
Description Capture antibody for cytokine 1, FITC-conjugated detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, capture antibody for cytokine 2, biotinylated detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA.
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