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Epidermal Transglutaminase (TG3) (hu)
Protein-glutamine gamma-glutamyltransferase E
Transglutaminase E, TG(E), TGE, TGase E
Gene name: TGM3
Transglutaminase E, TG(E), TGE, TGase E
Gene name: TGM3
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00038963
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold
| ID Covalab | opr0029 |
|---|---|
| Uniprot ID | Q08188 |
| Target | Transglutaminase-3, TGase-3 |
| Storage instructions | Store working aliquots at a minimum of -20°C Avoid repeated freezing and thawing Delivery at ambient temperature is possible |
| Description | His6-rhTG3 is based on the TGM3-allele from clone DKFZp686J0716 (isolated from different tissues), corrected by the insertion of the missing T at Position 435. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAALGV… . His6-rhTG3 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity. |
| Raised in | E. coli |
| Species | Hu |
| Form | Recombinant |
| Gene ID | 7053 |
| Labelling | His-Tag |
| Preparation method | His6-rhTG3 is a Ca2+-dependent enzyme.<br/>10 µl His6-rhTG3 (1 µg/µl)<br/>35 µl 50 mM Tris-HCl pH 8<br/>5 µl Dispase I (Roche) (0.2 µg/µl)<br/>Incubate for 20 min at 30°C<br/>Add 10 mM Ca2+ when measuring His6-rhTG3 activity. |
| Poids moléculaire | 78 kDa |
| Principe | His6-rhTG3 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG3 may also be used for immunoprecipitation and the detection of TG3 autoantibodies. |
| Pureté | > 95 % |
| Product type | Proteins |
| Applications | FA, IP |
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