Anti-Human pThr474 RIPK2 Rabbit Antibody

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Anti-Human pThr474 RIPK2 Rabbit Antibody
More Information
ID Covalab pab60466
Product type Primary antibodies
Clonality Polyclonal antibody
Raised in Rabbit
Immunogen Phosphopeptide corresponding to the pY474 site of human RIPK2.
Activity cross reaction Human and mouse RIPK2.
Species Human, Mouse
Form Whole Antiserum
Storage instructions Store at -20°C in appropriate aliquots to avoid multiple freeze-thaw cycles.
Target RIPK2 (phospho-Thr474)
Uniprot ID O43353
Gene ID 603455
Applications IHC, WB
Working dilutions Immunoblotting: use at a dilution of 1:500. Detection of RIPK2 in MDP-stimulated HEK293 cells (+) vs. non-stimulated HEK293 cells (-)
These are recommended dilutions.
Endusers should determine optimal dilutions for their applications.
Immunohistochemistry: use at a dilution of 1:100. Detection of active RIPK2 in human (top) and mouse (bottom) tissues. Non-IBD refers to colon sections from non-inflammatory bowel disease (IBD) patients; IBD refers to colon sections from ulcerative colitis (UC) patients. Mouse strain used: B6.129P2-I110tm1Cgn/J, a common model for IBD that presents with spontaneous colitis that is elevated by DSS (dextran sulfate sodium) exposure.
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