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Human IFNg / IL-10 Dual ELISpot set (sterile plate)
Interleukine-10 /
Interferon gamma, IFN gamma
Interferon gamma, IFN gamma
As low as
€323.10
In stock
Ref
00099535
Covalab Dual ELISpot Sets include capture and detection antibodies for both of the targeted cytokines, Streptavidin-Alkaline Phosphatase conjugated, anti-FITC HRP conjugated mAb, BSA, BCIP/NTB and AEC, blocking reagent and 1 sterile plate.
| ID Covalab | kit30023 |
|---|---|
| Principe | This Dual Colour ELISpot kit allows you to analysis the production of two cytokines simultaneously in the same well. |
| Applications | ELISpot |
| Detection method | Colorimetric |
| Labelling | AP, HRP |
| Activity cross reaction | Reacts with natural human IL-10 and IFN-g. Cross-reacts with both monkey proteins. Does not cross-react with other human cytokines nor with viral IL-10. |
| Species | Hu |
| Sample type | CD4+ cells that release cytokines upon stimulation. |
| Assay range | 1 - 2.5x105 cell/ 100 µl |
| Assay type | Sandwich |
| Assay duration | 3h30 after cell stimulation |
| Form | Sterile plates |
| Storage instructions | Store kit reagents between 2 and 8°C except uncoated plates which should be stored at RT. Immediately after use remaining reagents should be returned to cold storage (2 to 8°C). |
| Target | IFNg / IL-10 |
| Product type | Kits |
| Description | 96 well PVDF bottomed plates Capture Antibody for human IFNγ Capture Antibody for human IL-10 FITC conjugated detection antibody for IFNγ Biotinylated detection antibody for IL-10 Anti-FITC antibody HRP conjugate Streptavidin-Alkaline Phosphatase conjugate Bovine Serum Albumin (BSA) 50x Concentrated AEC substrate buffer 10x Concentrated dilution buffer for AEC substrate Ready to use BCIP/NBT substrate buffer |
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