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Apaf1 (C-Terminus) antibody
Apoptotic protease-activating factor 1, APAF-1
Gene name: APAF1, KIAA0413, CED4
Gene name: APAF1, KIAA0413, CED4
As low as
€587.00
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00097724
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
| ID Covalab | pab60018 |
|---|---|
| Product type | Primary antibodies |
| Clonality | Polyclonal antibody |
| Isotype | IgG |
| Raised in | Rabbit |
| Immunogen | Synthetic peptide, corresponding to amino acids 1158-1177 (C-terminus) of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid |
| Activity cross reaction | Reacts with human, mouse, and rat Apaf1 (130kDa). |
| Species | Hu, Ms, Rat |
| Labelling | None |
| Form | Purified |
| Preservative | NaN3 0.02% |
| Storage instructions | Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles. |
| Target | Apaf1 |
| Uniprot ID | O14727 |
| Gene ID | 317 |
| Applications | ELISA, ICC, IF, IHC, WB |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only: IHC (10 - 20 µg/ml) WB (1 - 10 µg/ml) ELISA (1:100 - 1:2000) |
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