Post-translational modifications of proteins play critical roles in the regulation and function of many biological processes. A common post-transcriptional modification of lysine is acetylation. The conserved aminoterminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs).
|Product type||Primary antibodies|
|Immunogen||Acetylated lysine-KLH conjugate.|
|Activity cross reaction||Reacts with proteins containing acetylated lysine residues. It does not recognize non-acetylated protein|
|Storage instructions||This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.|
|Applications||ELISA, ICC, IF, IHC - P, IP, WB|
|Working dilutions||Optimal dilutions should be determined by the end user.
The following are guidelines only:
ELISA (1-10 µg/ml with acetylated lysine-containing proteins on the solid phase)
WB ( 1 - 2 µg/ml)
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