Anti-RPA32 Antibody

RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34
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00123851
Anti-RPA32 Antibody
More Information
ID Covalab mab60783
Product type Primary antibodies
Clonality Monoclonal antibody
Clone 12F3.3
Isotype IgG2a
Raised in Mouse
Immunogen N-terminally 6x-His tagged fusion protein corresponding to full-length human RPA32 expressed in E. coli.
Activity cross reaction This antibody recognizes human and mouse RPA32. RPA (Replication Protein A) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32, and 14 kD. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination, and nucleotide excision repair. RPA32 is phosphorylated during the S-phase of the cell cycle, in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes.
Species Human, Mouse
Form Purified
Storage instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.
Target RPA32
Uniprot ID P15927
Applications ICC/IF, IP, WB
Working dilutions Immunoblotting,
Immunoprecipitation: use at 0.2-1 ug/mL.
Positive control: HeLa, Raji, and 3T3 cells and recombinant protein.
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