Methylated arginine antibody (7E6)

clone : 7E6
As low as €278.30
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Methylated arginine antibody (7E6)
More Information
ID Covalab mab0002-P
Product type Primary antibodies
Clonality Monoclonal antibody
Clone (7E6)
Isotype IgG1 / Kappa
Raised in Mouse
Immunogen Modified methyl arginine
Activity cross reaction Reacts with all structure of methylated arginine including free and bound. Cross reacts with free and bound NG - Monomethyl Arginine. No cross reaction with free or bound Arginine. Ability to fix complement : unknown
Species All Sp.
Labelling None
Form Purified (protein A)
Preservative None
Storage instructions Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Target methylated arginine monomethyl arginine (MMA), asymetric dimethyl arginine (ADMA) and symetric dimethyl arginine (SDMA)
Applications ChIP, ELISA, IP, WB
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
- ChIP : 1/100 to 1/10 000
- WB : 1/500 to 1/5 000
Publication * SR Green. Regulation of Citric Acid Cycle Enzymes and Related Pathways in the Skeletal Muscle of Hibernating Richardson’s Ground Squirrels, Urocitellus Richardsonii. Thesis Nov 2021 (WB)
* SR Green et al. BBA - Proteins and Proteomics. 2020 May 20
* CL Childers et al. J Comp Physiol B. 2019 Jan 10
* Mattice JJL. Regulation of glutathione-based antioxidant defenses in response to dehydration stress in the African clawed frog, Xenopus laevis. Thesis 2018
* Alban C et al. Uncovering the protein lysine and arginine methylation network in Arabidopsis chloroplasts. PLoS One. 2014 Apr 18 :9(4):e95512. WB, plants
* Abboud J and Storey KB. Novel control of lactate dehydrogenase from the freeze tolerant wood frog: role of posttranslational modifications. PeerJ 2013 1:e12: WB
* Duong F. et al. Hepatitis C virus inhibits interferon signaling through up-regulation of protein phosphatase 2A. Gastroenterology. 2004 Jan:126(1):263-77.
* Ong S.E. et al. Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat Methods. 2004 Nov:1(2):119-26.
* Mowen K.A. et al. Arginine Methylation of STAT1 modulates IFNalpha/beta-induced transcription. Cell. (2004), Nov 24, 119(5),587-590.
* Fabbrizio E. et al. Identifying and quantifying in vivo methylation sites by heavy methyl SILAC. Nat Methods. 2004 Nov:1(2):119-26.
* Li H. et al. Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase. J Biol Chem. 2002 Nov 22:277(47):44623-30.
* Rho J. and al. The arginine-1493 residue in QRRGRTGR1493G motif IV of the hepatitis C virus NS3 helicase domain is essential for NS3 protein methylation by the protein arginine methyltransferase 1. J Virol. 2001 Sep:75(17):8031-44.
* Zhu W. et al. Arginine methylation of STAT1 regulates its dephosphorylation by T cell protein tyrosine phosphatase. J Biol Chem. 2002 Sep 27:277(39):35787-90.
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