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Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit : FXIII-CovTest
Protein-glutamine gamma-glutamyltransferase A chain
Transglutaminase A chain
Factor XIII, FXIII, F XIII, Factor 13
Gene name : F13A1, F13A
Transglutaminase A chain
Factor XIII, FXIII, F XIII, Factor 13
Gene name : F13A1, F13A
As low as
€488.80
In stock
Ref
00028996
A colorimetric microassay for quantification of Factor XIII activity
| ID Covalab | opr0037 |
|---|---|
| Principe | The F XIII-Covtest uses biotinylated F11 peptide (Biotin-pepF11) as the first substrate (amine-acceptor/acyl-donor) and an amine-donor/acyl-acceptor as a second substrate. In the presence of activated F XIII (suspected to be contained in the samples), the γ carboxamide of the glutaminyl residue of the biotin-pepF11 is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the FXIII activity and is revealed by a peroxidase colorimetric reaction. |
| Applications | FA |
| Detection method | Colorimetric |
| Labelling | HRP |
| Activity cross reaction | Measurement of activated plasmatic Transglutaminase XIII (FXIIIa) with low or no interference with other TGs isoforms. |
| Species | All Sp. |
| Sample type | Blood plasma |
| Assay range | 0.01 mU/ml to 0.5 mU/ml |
| Assay type | Colorimetric Microassay |
| Assay duration | 45 min |
| Storage instructions | DTT and SAv-HRP enzyme at -20°C. Other reagents at +4°C. |
| Target | Plasmatic transglutaminase |
| Uniprot ID | P00488 |
| Publication | - Hitomi K, Kitamura M, Alea MP, Ceylan I, Thomas V, El Alaoui S. A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. Anal Biochem. 2009 Nov 15:394(2):281-3. |
| Product type | Kits |
| Description | Microtiter strips coated with amine substrate DTT EDTA (Negative Control) Reaction Buffer (Biotin-pepF11/CaCl2) Enzyme Tracer (SAv-HRP) Wash Buffer 10X Diluent Buffer 10X HRP Substrate Blocking Reagent |
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