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Transglutaminase Colorimetric Microassay Kit
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€497.30
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00028962
A colorimetric microassay for quantification of transglutaminase activity.
| ID Covalab | opr0001 |
|---|---|
| Principe | The TG-Covtest uses CBZ-Gln-Gly as the first substrate (amine acceptor) and biotin cadaverine as second (amine donor). Tgase (suspected to be contained in the samples) induced the incorporation of biotinylated cadaverine to gamma carboamyde of the glutaminyl residue of the dipeptide. The quantity of biotin incorporated is related to the transglutaminase activity and is revealed by a peroxidase colorimetric reaction. |
| Applications | FA |
| Detection method | Colorimetric |
| Labelling | HRP |
| Activity cross reaction | Measurement of Transglutaminase activity in biological samples. |
| Species | All Sp. |
| Sample type | Biological fluids, cell and tissus lysates, recombinant Tgase |
| Assay type | Colorimetric Microassay |
| Assay duration | 1h 30 min |
| Storage instructions | Store biotin-cadaverine and enzyme tracer and opr0036 at -20°C Other reagents at +4°C |
| Target | Transglutaminases |
| Publication | Espitia Pinzón N et al. J Neuroinflammation. 2017 Dec 28,14(1):260. * Luo R et al. J Am Heart Assoc. 2016 Jun 30,5(7). * van Strien ME et al. Brain Behav Immun. 2015 Jun 29. pii: S0889-1591(15)00232-9. * Yu J et al. J Biol Chem. 2015 Jun 17. * Roncoroni L et. J Transl Med. 2013 Apr 8,11(1):91. * Mackay DS et al. J Nutr Biochem. 2011 Nov 30. * Barathi VA et al. T. PLoS One. 2011 Apr 6,6(4):e18326. * van den Akker J et al. Amino Acids. 2012 Feb,42(2-3):961-73. * Elli L et al. Lab Invest. 2011 Mar:91(3):452-61. |
| Product type | Kits |
| Description | 3x96-well plates, Negative control, Biotin-cadaverine, Enzyme tracer, Diluent buffer, HRP substrate, Chromogen, Blocking reagent, Positive control (opr0036) |
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