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Bacterial Pro-transglutaminase (mTG)
Protein-glutamine-γ-glutamyltransferase, Protein-glutamine gamma-glutamyltransferase
microbial transglutaminase, BTG
Transglutaminase, TGase
Gene name: MTG
microbial transglutaminase, BTG
Transglutaminase, TGase
Gene name: MTG
As low as
€355.30
In stock
Ref
00109858
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These
enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
| ID Covalab | pro0004 |
|---|---|
| Uniprot ID | P81453 |
| Target | Bacterial Pro-transglutaminase (mTG) |
| Preservative | None |
| Storage instructions | Store working aliquots at a minimum of -20°C Avoid repeated freezing and thawing Delivery at ambient temperature is possible |
| Description | No material of animal origin is used within production process. Gene derived from Streptomyces mobaraensis. |
| Raised in | E. coli |
| Species | Str. |
| Form | Recombinant |
| Preparation method | Activation:<br/>10 μl His6-rProTG (1 μg/μl)<br/>35 μl 50 mM Tris-HCl pH 8<br/>5 μl Dispase II (Roche) (0.2 μg/μl)<br/>Incubate for 20 min at 30°C |
| Poids moléculaire | 43 kDa |
| Principe | mTG can be used for labelling, immobilisation, conjugation and modification of proteins. |
| Pureté | > 90% [SDS-PAGE and Coomassie staining] |
| Product type | Proteins |
| Applications | FA |
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