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Bacterial Transglutaminase (mTG)
Protein-glutamine-γ-glutamyltransferase, Protein-glutamine gamma-glutamyltransferase
microbial transglutaminase, BTG
Transglutaminase, TGase
Gene name: MTG
microbial transglutaminase, BTG
Transglutaminase, TGase
Gene name: MTG
As low as
€355.30
In stock
Ref
00012429
Transglutaminases are a family of enzymes that catalyze the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains. These enzymes catalyze the acyl transfer reaction between the γ-carboxyamide groups of peptidebound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine. The resulting crosslink is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
ID Covalab | opr0054 |
---|---|
Uniprot ID | P81453 |
Target | Bacterial transglutaminase |
Preservative | None |
Storage instructions | Store working aliquots at a minimum of -20°C Avoid repeated freezing and thawing Delivery at ambient temperature is possible |
Description | Microbial (bacterial) transglutaminase is recombinantly produced in the inactive zymogenic form (Pro-MTG) in E. coli in a shaker flask fermentation. To achieve the production of larger amounts of Pro-MTG, a stirred tank fermentation process was developed. For this purpose, the expression plasmid was changed. Essentially, an expression plasmid carrying a kanamycin-resistance gene is used instead of the former expression plasmid, which contained a β-lactamase-gene. The fermentation medium was changed, however, no animal derived media components are used in the stirred tank fermentation protocol. |
Raised in | E. coli |
Species | Str. |
Form | Recombinant |
Poids moléculaire | 38 kDa |
Principe | mTG can be used for labelling, immobilisation, conjugation and modification of proteins. |
Pureté | > 95 % |
Product type | Proteins |
Applications | FA |
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