Expert in peptide Engineering

Peptide Synthesis Service

NEW!  Request a quotation online!  NEW!

Custom peptides are important tools in drug discovery and proteomic research. They can be used for example as bioactive molecules (such as immunogen, enzymatic substrate or inhibitor), or as probes when studying protein structure and function. Thanks to our expertise in the fields of peptide design, synthesis and modification, we provide a high-quality peptide synthesis service, either standard peptide synthesis or express peptide synthesis, as well as a wide range of chemical modifications.

Our peptides are synthesised using solid-phase Fmoc chemistry and fully automated multiple peptide synthesisers. We provide synthetic peptides with a large range of purity levels, amounts and N-terminal, C-terminal or side chain chemical modifications.

Reversed phase HPLC and mass spectrometry data are supplied with every peptide to verify peptide composition and purity. The production time may vary depending on peptide quantity and purity purchased, and is usually two to three weeks.

Peptide Customisation

Length

We can produce peptides as short as 3 and as long as 90 residues. As a rule, 15 residues are sufficient to develop polyclonal or monoclonal antibodies. Peptides longer than 90 residues may be synthesised upon specific conditions. Please for more details if you have any specific requirements.

Quantity

Peptide Chemistry

Our equipment allows us to produce down to 1 milligram and up to several grams of peptide.

Purity

We propose several purity levels, ranging from moderate (>70%) to high (>95%) by 5% increments. Very high purity (>98%) level may be reached on request for specific applications. Peptides are also available as crude.

We recommend the following purity levels for peptides used in common applications:

  • Immunograde peptides (>70%, >75% or >80% purity): polyclonal antibody development, immunoassay for antibody titration…
  • Biochemistry grade peptides (>85% or >90% purity): biochemical applications (e.g. qualitative enzyme-substrate studies or production of affinity resins)…
  • High purity peptides (>95% or >98% purity): quantitative results (e.g. enzymology or biological assays), NMR studies…

Special Residues

In addition to the conventional proteogenic amino acids, we can incorporate special forms of these residues such as α- or β-homo amino acids or D-form residues, as well as unnatural residues such as citrulline or pyroglutamine. For more details, please consult our complete list of available special residues. If you don’t find what you are looking for, please .

Peptide Modification

Chemical Modifications

Thanks to our expertise in peptide chemistry, we can also bring very precise chemical modifications such as:

  • addition of one or several functional groups (acetylation, phosphorylation…)
  • labelling with a probe
  • coupling to a carrier protein
  • alteration of structure (cyclisation, disulphide bonds…)

For more details, please consult our complete list of available modifications.

Recommendations

Solubility

Lyophilised peptides

All peptides are shipped lyophilised. The first solvent of choice is deionised water. However, solubilising peptides can reveal itself difficult, particularly in the case of very high hydrophobicity. Sonication may help dissolve the peptide. In the following cases, we recommend solubilising a small quantity of the peptide first to determine the optimal solubilisation conditions:

  • Basic peptides containing predominantly basic residues (Arg, Lys, His): add 10% acetic acid or 0.1% TFA (trifluoroacetic acid) to increase the solubility. Increase the concentration of acid stepwise if the peptide does not dissolve.
  • Acidic peptides containing predominantly acidic residues (Asp, Glu): add 1% ammonium hydroxide or 10% ammonium bicarbonate to help solubilise the peptide.
  • Hydrophobic (Trp, Phe, Leu, Ile, Met, Val and Tyr) or neutral peptides: add acetonitrile, isopropanol, DMF or DMSO (from 5 to 50%) stepwise. Please note that these solvents may have a damaging effect on your experiments. For peptides with secondary structures, it may be necessary to add chaotropic agents such as urea or guanidium-HCl.

Storage

For maximum stability we recommend storing the lyophilised peptide at –20°C. At this temperature, peptides should remain stable for several years. Peptides in solution should be aliquoted and stored at –20°C to avoid repeated freeze-thaw cycles.

Hygroscopic peptides should be brought to room temperature before opening the vial. The vial should be re-sealed as soon as possible to prevent absorbtion of water from the atmosphere which can reduce the peptide stability.

Peptides containing Trp, Met and Cys are susceptible to oxidation and should be stored under nitrogen or argon atmosphere. Solutions of these peptides should be degassed and stored at –70°C. We recommend the same procedure for peptides containing Gln and Asn which are prone to deamidation.

Do not hesitate to see our Frequently Asked Questions section or feel free to contact us if you need more information about peptides.

Quality control

HPLC - MS analyses

We guarantee the quality of all peptides produced. A control is performed routinely on each synthesised peptide by High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) analyses.

◁ Back to Services page

Newsletter Receive news, promotions and offers in your e-mail box