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Twist-1 antibody (3E10)

Twist-related protein 1
Class A basic helix-loop-helix protein 38, bHLHa38
Gene name : TWIST1, BHLHA38, TWIST
This transcriptional regulator inhibit myogenesis and represses the expression of pro-inflammatory cytokines.

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Price From: €164.00
Ref : 00012948

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Product Code mab0089-P
Clone (3E10)
Immunogen Synthetic peptide derived from Nter domain of human Twist-1 protein
Target Specificity Reacts with human TWIST1 protein. Cross reacts with mouse, rat and other species. Not cross reacts with Twist-2
Name Twist-related protein 1
Uniprot ID Q15672
Gene ID 7291
Print datasheet
Product type Primary antibodies
Clonality Monoclonal antibody
Isotype IgG1 / Kappa
Produced in Mouse
Species Human
Labelling None
Appearance Lyophilized powder
Conc. / Activity 1 mg/ml
Form Purified (protein A)
Constituents Tris 0,1M, glycine 0,1M, sucrose 2%
Preservatives None
Recommendations Must be reconstituted in distilled water.
Storage Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Applications Western Blot, Immunohistochemistry, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC (1:100 - 1:1000)
WB (1:1000 - 1:5000)
Research areas Cancer,Developmental biology,Epigenetic,Neurology
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* Selmi A et al. TWIST1 is a direct transcriptional target of MYCN and MYC in neuroblastoma. Cancer Lett. 2014 Dec 1. pii:S0304-3835(14)00741-1.

WB - Twist-1 antibody (3E10 )<br/>(mab0089-P)<br/>Anti-Twist-1 antibody WB staining of nuclear (2-4) or cytoplasmic extract (6-10) from 293T cells transfected with:<br/>2,5,8 :  empty vector<br/>3,6,9 : Twist1-Flag vector<br/>4,7,10: Twist2-Flag vector<br

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