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Transglutaminase Colorimetric Microassay Kit

A colorimetric microassay for quantification of transglutaminase activity.

If you want to order offline

orders@covalab.com - tel: +33 (0) 437 654 230 - fax: +33 (0) 437 289 416

€360.00
Available
Ref : 00028962

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Product Code opr0001
Target Specificity Measurement of Transglutaminase activity in biological samples.
Constituents 3x96-well plates,
Negative control,
Biotin-cadaverine,
Enzyme tracer,
Diluent buffer,
HRP substrate,
Chromogen,
Blocking reagent
Name Transglutaminases
Print datasheet
Principle The TG-Covtest uses CBZ-Gln-Gly as the first substrate (amine acceptor) and biotin cadaverine as second (amine donor). Tgase (suspected to be contained in the samples) induced the incorporation of biotinylated cadaverine to gamma carboamyde of the glutaminyl residue of the dipeptide. The quantity of biotin incorporated is related to the transglutaminase activity and is revealed by a peroxidase colorimetric reaction.
Applications Functional Assay
Detection method Colorimetric
Labelling Horseradish peroxidase (HRP)
Species All Species
Sample type Biological fluids, cell and tissus lysates, recombinant Tgase
Assay Type Colorimetric Microassay
Assay duration 1h 30 min
Recommendations A colorimetric microassay for quantification of transglutaminase activity.
The TG-CovTest uses CBZ-Gln-Gly as the first substrate (amine acceptor) and biotin-cadaverine as second (amine donor). In the 1st step, samples suspected of containing TG are i
Storage Store biotin-cadaverine and enzyme tracer and opr0036 at -20°C
Other reagents at +4°C
Research areas Post translational modifications,Transglutaminase
Product type Kits
Description 3x96-well plates,
Negative control,
Biotin-cadaverine,
Enzyme tracer,
Diluent buffer,
HRP substrate,
Chromogen,
Blocking reagent,
Positive control (opr0036)
Print datasheet
* Luo R et al. J Am Heart Assoc. 2016 Jun 30,5(7).
* van Strien ME et al. Brain Behav Immun. 2015 Jun 29. pii: S0889-1591(15)00232-9.
* Yu J et al. J Biol Chem. 2015 Jun 17.
* Roncoroni L et. J Transl Med. 2013 Apr 8,11(1):91.
* Mackay DS et al. J Nutr Biochem. 2011 Nov 30.
* Barathi VA et al. T. PLoS One. 2011 Apr 6,6(4):e18326.
* van den Akker J et al. Amino Acids. 2012 Feb,42(2-3):961-73.
* Elli L et al. Lab Invest. 2011 Mar:91(3):452-61.

Transglutaminase Colorimetric Microassay Kit

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