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TIA1 (Internal) antibody

Nucleolysin TIA-1 isoform p40
RNA-binding protein TIA-1
T-cell-restricted intracellular antigen-1, TIA-1
TIA1 cytotoxic granule-associated RNA binding protein
Gene name: TIA1
TIA-1 is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.

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Ref : 00021018

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Product Code pab72947
Immunogen Synthetic peptide C-PKSTYESNTKQ from an internal region (aa189-199) of human TIA1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Opossum, Chicken, Xenopus (100%), Hamster (91%), Horse, Pig, Turkey, Pufferfish (82%).
Target Specificity Reacts with TIA1 from human, monkey, mouse, rat, bat, bovine, dog, rabbit, chicken, Xenopus. This antibody is expected to recognize both reported isoforms (NP_071320.1 and NP_071505.1).
Name TIA1
Uniprot ID P31483
Gene ID 7072
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Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Goat
Species Human, Mouse, Rat, Canine, Chicken, Xenopus
Labelling None
Appearance Liquid
Conc. / Activity 0.5 mg/ml
Form Antigen affinity purified
Constituents TBS, pH 7.3, BSA 0.5%
Preservatives NaN3 0.02%
Storage Store at -20°C. Minimize freezing and thawing.
Applications Western Blot, IHC - Paraffin sections, Immunofluorescence, Immunocytochemistry, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only:
ELISA (1:128000)
IHC-P (3.75 µg/ml)
WB (0.1 - 0.3 µg/ml)
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IHC - TIA1 (Internal) antibody<br/>(pab72947)<br/>Anti-TIA1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 µg/ml.

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