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Specific Tissue Transglutaminase (tTG/TGase2) Colorimetric Microassay Kit : TG2-CovTest

Protein-glutamine gamma-glutamyltransferase 2
Tissue transglutaminase
Transglutaminase C (TG(C)), TGC, TGase C
Transglutaminase H, TGase H
Gene name: TGM2
A colorimetric microassay for quantification of Tissue Tranglutaminase activity

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Product Code opr0030
Target Specificity Measurement of Transglutaminase-2 activity in biological samples with low or no interference with other TGs isoforms.
Constituents Microtiter strips with covalently bound spermine
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pepT26/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Positive contr
Name Transglutaminase-2, TGase-2
Print datasheet
Principle The TG2-Covtest uses biotinylated T26 peptide (Biotin-pepT26) as the first substrate (amine-acceptor/acyl-donor) and an amine-donor/acyl-acceptor as a second substrate.
In the presence of Tgase-2 (suspected to be contained in the samples), the γ carboxamide of the glutaminyl residue of the biotin-pepT26 is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the Tgase-2 activity and is revealed by a peroxidase colorimetric reaction.
Applications Functional Assay
Detection method Colorimetric
Labelling Horseradish peroxidase (HRP)
Species All Species
Sample type Biological fluids, cell and tissus lysates, recombinant Tgase-2
Assay range 2.5 - 40 µU/ml
Assay Type Colorimetric Microassay
Assay duration 45 min
Recommendations The TG2-Covtest uses biotinylated T26 peptide (Biotin-pepT26) as the first substrate (amine-acceptor/acyl-donor) and spermine as second substrate (amine-donor/acyl-acceptor).
Samples suspected of containing TG2 are incubated with calcium, dithiothreit
Storage DTT, SAv-HRP enzyme and opr0036 at -20°C.
Other reagents at +4°C.
Uniprot ID P21980
Research areas Post translational modifications,Transglutaminase
Product type Kits
Sensitivity 1.25 µU/ml
Description Microtiter strips coated with amine substrate
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pepT26/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Positive control
Print datasheet
* Meshram DD et al. Eur J Pharmacol. 2017.09.035
* Carbone C et al. Amino Acids. 2016 Oct 19.
* Luo R et al. J Am Heart Assoc. 2016 Jun 30,5(7).
* Király R et al. Amino Acids. 2016 Jan,48(1):31-40.
* Liu C et al. Hypertension. 2014 Feb,63(2):353-61.
* Kumar A et al. BMC Cancer. 2014 Apr 11;14:256.
* Caffarel MM et al. J Pathol. 2013 Oct,231(2):168-79.
* Wang Z and Griffin M. PLoS One. 2013,8(3):e57017.
* Perez Alea M et al. Amino Acids. 2013 Jan,44(1):245-50.
* Hong GU et al. Cell Signal. 2013 Jun,25(6):1514-25.
* Perez Alea M et al. Anal Biochem. 2009 Jun 15,389(2):150-6.

Assay specificity for TG2<br/>(opr0030)

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