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Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit : FXIII-CovTest

Protein-glutamine gamma-glutamyltransferase A chain
Transglutaminase A chain
Factor XIII, FXIII, F XIII, Factor 13
Gene name : F13A1, F13A
A colorimetric microassay for quantification of Factor XIII activity

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Price From: €360.00
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Product Code opr0037
Target Specificity Measurement of activated plasmatic Transglutaminase XIII (FXIIIa) with low or no interference with other TGs isoforms.
Constituents Microtiter strips with covalently bound spermine
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pepF11/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Name Plasmatic transglutaminase
Print datasheet
Principle The F XIII-Covtest uses biotinylated F11 peptide (Biotin-pepF11) as the first substrate (amine-acceptor/acyl-donor) and an amine-donor/acyl-acceptor as a second substrate. In the presence of activated F XIII (suspected to be contained in the samples), the γ carboxamide of the glutaminyl residue of the biotin-pepF11 is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the FXIII activity and is revealed by a peroxidase colorimetric reaction.
Applications Functional Assay
Detection method Colorimetric
Labelling Horseradish peroxidase (HRP)
Species All Species
Sample type Blood plasma
Assay range 0.01 mU/ml to 0.5 mU/ml
Assay Type Colorimetric Microassay
Assay duration 45 min
Recommendations Pre-treatment with thrombin (0.5-1 IU/ml) to obtain activated FXIII (FXIIIa) is necessary to measure the activity using FXIII CovTest.
Storage DTT and SAv-HRP enzyme at -20°C.
Other reagents at +4°C.
Uniprot ID P00488
Research areas Post translational modifications,Transglutaminase
Product type Kits
Description Microtiter strips coated with amine substrate
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pepF11/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Print datasheet
- Hitomi K, Kitamura M, Alea MP, Ceylan I, Thomas V, El Alaoui S. A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. Anal Biochem. 2009 Nov 15:394(2):281-3.

Specific Plasmatic Transglutaminase (Factor XIII/ FXIII) Colorimetric Microassay Kit : FXIII-CovTest

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