Close Forward an order
By clicking this button, you can send a message to your purchasing manager to ask her/him to order this product. All information needed about our company and the product will be automatically attached. You will also have the opportunity to ask us for more information about the product. If so, your request will be forwarded to our technical service.

Captcha

* Required Fields

Back to products list

Specific Microbial /Bacterial Transglutaminase (MTG) Colorimetric Microassay Kit : MTG-CovTest

microbial transglutaminase, MTg, Tg Bact
A colorimetric microassay for quantification of Bacterial Transglutaminase activity

If you want to order offline

orders@covalab.com - tel: +33 (0) 437 654 230 - fax: +33 (0) 437 289 416

€360.00
Available
Ref : 00029002

* Required Fields

Product Code opr0053
Target Specificity Measurement of Bacterial transglutaminase (MTGase) with low or no interference with other TGs isoforms.
Constituents Microtiter strips with covalently bound spermine
DTT
EDTA (Negative Control)
Reaction Buffer (Biotin-pep/CaCl2)
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Name Bacterial transglutaminase
Print datasheet
Principle The MTG-CovTest uses biotinylated peptide originated from the F-helix of myoglobin (Biotin-pepM) as the first substrate (amine-acceptor/acyl-donor) aand an amine-donor/acyl-acceptor as a second substrate. In the presence of MTgase (suspected to be contained in the samples), the gamma carboxamide of the glutaminyl residue of the Biotin-pepM is incorporated into the amine substrate to form biotinylated isopetide bound. The quantity of biotin incorporated is related to the transglutaminase activity and is revealed by a peroxidase colorimetric reaction.
Applications Functional Assay
Detection method Colorimetric
Labelling Horseradish peroxidase (HRP)
Species Streptomyces
Assay Type Colorimetric Microassay
Assay duration 45 min
Recommendations The MTG-CovTest uses biotinylated peptide originated from the F-helix of myoglobin (Biotin-pepM) as the first substrate (amine-acceptor/acyl-donor) and spermine as second substrate (amine-donor/acyl-acceptor).
Samples suspected of containing MTgase a
Storage Store SAv-HRP enzyme at -20°C, other reagents at +4°C.
Research areas Post translational modifications,Transglutaminase
Product type Kits
Description Microtiter strips coated with amine substrate
Reaction Buffer ( Biotin-pepM )
Wash Buffer 10X
Diluent Buffer 10X
Enzyme Tracer (SAv-HRP)
Enzyme Tracer Diluent buffer 10X
HRP Substrate
Blocking Reagent
Print datasheet

Specific Microbial /Bacterial Transglutaminase (MTG) Colorimetric Microassay Kit : MTG-CovTest

Newsletter Receive news, promotions and offers in your e-mail box