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Specific Keratinocyte Transglutaminase (Tgase-1) Colorimetric Microassay Kit : TG1-CovTest

Protein-glutamine gamma-glutamyltransferase K
Epidermal TGase
Gene name: TGM1, KTG
A colorimetric microassay for quantification of Keratinocyte Transglutaminase (TG1) activity

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Product Code opr0038
Target Specificity Measurement of Transglutaminase-1 activity in biological samples with low or no interference with other TGs isoforms.
Constituents Microtiter strips with covalently bound spermine
DTT
EDTA (Negative Control)
Reaction Buffer A (Biotin-pepK5/CaCl2)
Reaction Buffer B
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Rea
Name Transglutaminase-1 (TGase-1)
Print datasheet
Principle The TG1-Covtest uses biotinylated K5 peptide (Biotin-pepK5) as the first substrate (amineacceptor/ acyl-donor) and spermine as second substrate (amine-donor/acyl-acceptor). In the presence of Tgase-1 (suspected to be contained in the samples), spermine is incorporated into the gamma carboxamide of the glutaminyl residue of the biotin-pepK5. The quantity of biotin incorporated is related to the Tgase-1 activity and is revealed by a peroxidase colorimetric reaction.
Applications Functional Assay
Detection method Colorimetric
Labelling Horseradish peroxidase (HRP)
Species All Species
Sample type Biological fluids, cell and tissue lysates, recombinant Tgase-1
Assay range 0.003 - 0.15 mU/ml
Assay Type Colorimetric Microassay
Assay duration 45 min
Recommendations The TG1-Covtest uses biotinylated K5 peptide (Biotin-pepK5) as the first substrate (amineacceptor/ acyl-donor) and spermine as second substrate (amine-donor/acyl-acceptor).
Samples suspected of containing TG1 are incubated with calcium, dithiothreitol
Storage DTT, Reaction Buffer B and SAv-HRP enzyme at -20°C.
Other reagents at +4°C.
Uniprot ID P22735
Gene ID 7051
Research areas Post translational modifications,Transglutaminase
Product type Kits
Description Microtiter strips with covalently bound spermine
DTT
EDTA (Negative Control)
Reaction Buffer A (Biotin-pepK5/CaCl2)
Reaction Buffer B
Enzyme Tracer (SAv-HRP)
Wash Buffer 10X
Diluent Buffer 10X
HRP Substrate
Blocking Reagent
Print datasheet
Hitomi K, Kitamura M, Alea MP, Ceylan I, Thomas V, El Alaoui S. A specific colorimetric assay for measuring transglutaminase 1 and factor XIII activities. Anal Biochem. 2009 Nov 15:394(2):281-3.

Specific Keratinocyte Transglutaminase (Tgase-1) Colorimetric Microassay Kit : TG1-CovTest

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