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Tissue Transglutaminase (His6-rhTG2)

rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. It may also be used for immunoprecipitation.

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€150.00
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Ref : 00024748

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Product Code opr0036
Uniprot ID P21980
Name Transglutaminase-2, TGase-2
Print datasheet
Appearance White lyophilized solid.
Constituents 20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM DTT, 10 mg/ml Sucrose
Storage Store lyophilized product at -80°C
Avoid repeated freezing and thawing
Delivery at ambient temperature is possible.
Description His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV….
His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 90 % purity.
Produced in Escherichia coli
Species Human
Form Recombinant
Gene ID 7052
Labelling His-Tag
Preparation method The Transglutaminase is activated with 10 mM Ca2+, due to the precipitation of Calcium Phosphate a buffer exchange (e. g. Tris-Buffer) prior to activation is highly recommended
PDB ID 1FAU
Molecular weight 78 kDa
Principle His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines. It may also be used for immunoprecipitation.
Purity > 95 %
Recommendations Add distilled water (see specific datasheet for the exact volume). Rotate vial gently until solid dissolves.
Product type Proteins
Applications Immunoprecipitation, Functional Assay
Research areas Transglutaminase
Print datasheet

Tissue Transglutaminase (His6-rhTG2)

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