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Protein-arginine deiminase type-3 (PADI3) antibody

Protein-arginine deiminase type-3
Protein-arginine deiminase type III
Gene nmae : PADI3, PDI3
PAD3
Catalyzes the deimination of arginine residues of proteins. Involved in protein citrullination.

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Product Code pab0198-P
Immunogen A 15 residue synthetic peptide derived from N-terminal domain of human PADI3 protein
Target Specificity Reacts with 75 kDa PADI3 protein from mouse, human and rat. No cross reaction with the other isoforms.
Name Protein-arginine deiminase type-3
Uniprot ID Q9ULW8
Gene ID 51702
Print datasheet
Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human, Mouse, Rat
Labelling None
Appearance Lyophilized powder
Conc. / Activity 1 mg/ml
Form Purified (protein A)
Constituents Tris 0,1M, glycine 0,1M, sucrose 2%
Preservatives None
Recommendations Must be reconstituted in distilled water.
Storage Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Immunoprecipitation
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA (1:5000 - 1:20000)
IHC/ ICC/ IF (1:50 - 1:500)
IP (1:20 - 1:100)
WB (1:200 - 1:2000)
Research areas Chromatin,Post translational modifications,Signal transduction
Print datasheet
*U KP, Subramanian V, et al. Modulation of calcium-induced cell death in human neural stem cells by the novel peptidylarginine deiminase-AIF pathway. Biochim Biophys Acta. 2014 Jun:1843(6):1162-71. (ICC, WB, IP, Hu)
*Mechin M.C. et al.The peptidylarginine deiminase expressed in human epidermis differ in their substrate specificities and subcellular locations. Cell Mol Life Sci. 2005;62:1984-95. (Hu)
*Kanno T. et al. Human peptidylarginine deiminase type III : molecular cloning and nucleotide sequence of the cDNA, properties of the recombinant enzyme, and immunohistochemical localization in human skin. J Invest Dermatol.2000;115:813-23.(WB, IF, Hu)

Protein-arginine deiminase type-3 (PADI3) antibody

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