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Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
MAD3/BUB1-related protein kinase, hBUBR1
Mitotic checkpoint kinase MAD3L
Protein SSK1
Gene name: BUB1B, BUBR1, MAD3L, SSK1
BUB1beta, Bub1A, MVA1
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
Defects in BUB1B are the cause of premature chromatid separation trait (PCS).
Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome type 1 (MVA1).

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Product Code mab50064
Clone 8G1
Immunogen A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1)
Target Specificity Reacts with human and mouse hBUBR1 protein.
Name Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Uniprot ID O60566
Gene ID 701
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Product type Primary antibodies
Clonality Monoclonal antibody
Isotype IgG1 / Kappa
Produced in Mouse
Species Human, Mouse
Labelling None
Appearance Liquid
Conc. / Activity 0,6 mg/ml
Form Purified (protein G)
Preservatives None
Storage Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Applications Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Immunoprecipitation
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
WB (1:2000 - 1:5000)
FC (1 µg per million cells)
ICC/IF (1:100)
IP (1:10 - 1:500)
Research areas Cancer,Kinases and phosphatases,Post translational modifications
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ICC/IF - BuBR1 antibody (8G1)<br/>(mab50064)<br/>Anti-BuBR1 antibody ICC/IF staining of asynchronous cycling population of human cells (U2OS)

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