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LC3B antibody

Microtubule-associated proteins 1A/1B light chain 3B
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
MAP1 light chain 3-like protein 2
MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B
Microtubule-associated protein 1 light chain 3 beta
Gene name: MAP1LC3B, MAP1ALC3
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

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Ref : 00030609

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Product Code pab50206
Immunogen Synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100)
Target Specificity Reacts with LC3B from human, mouse, rat, bacteria, bovin, syrian hamster, porcine and zebrafish.
Name LC3B
Uniprot ID Q9GZQ8
Gene ID 81631
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Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human, Mouse, Rat, Bovine, Zebrafish, Bacteria
Labelling None
Appearance Liquid
Conc. / Activity 1 mg/ml
Form Antigen affinity purified
Constituents Tris-glycine, 150 mM NaCl
Preservatives NaN3 0.05%
Storage Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Immunoprecipitation
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
WB (0.5 - 2 µg/ml)
ICC/IF (1:200)
IHC 1(:200 - 1:400)
IP (20 µg/500 µg of protein)
Research areas Autophagy,Cardiac and vascular biomarkers,Neurology
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WB - LC3B antibody<br/>(pab50206)<br/>Anti-LC3B antibody WB staining of mouse ES cell lysate.

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