Labelling - Our Services

Pre-labelling study and control

Before performing the labelling, several parameters should be optimised. Adverse effects can indeed be observed on the antibodies immunoreactivity. This first labelling must be considered as a feasibility study. To determine the best coupling conditions, each project includes the following steps :

conjugation of antibodies with a fluorochrome or biotin at three different concentrations
conjugation of antibodies with an enzyme at one concentration (according to the literature)
control of the antibodies immunoreactivity before and after conjugation (the immunogen is required to perform this step, please for more details)
shipment of conjugated products samples to your laboratory so that you can determine the dye/antibody ratio which yields the best signal.

According to the nature of the dye, different methods are carried out:

fluorochromes and biotin are coupled to 100 μg of antibody via an activation by isothiocyanate or a succinimidyl ester, respectively
ALP is coupled to 100 μg of antibody using the technique set up by Avrameas et al.
HRP is coupled to 100 μg of antibody using the technique set up by Nakane et al.

Labelled antibodies are provided in a phosphate buffer saline containing BSA, thimerosal or sodium azide, glycerol 50%.

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Labelled antibody production

Following the feasibility study and upon your confirmation according to the results of your tests, the optimal dye/ antibody ratio will be used to produce conjugated antibodies in higher amount.

Quality control:

estimation of the molar ratio (for fluorochrome- and biotin-conjugated antibodies),
control of the immunoreactivity of the antibodies after labelling (the immunogen is required to perform this step, please for more details),
study of the enzymatic activity (for enzyme-conjugated antibodies).

This service is performed for a minimal quantity of 500 μg of antibody.

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