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Hypoxia inducible factor-2 alpha (HIF2-alpha) antibody

Endothelial PAS domain-containing protein 1 (EPAS-1)
Basic-helix-loop-helix-PAS protein MOP2
Class E basic helix-loop-helix protein 73 (bHLHe73)
HIF-1-alpha-like factor (HLF)
Hypoxia-inducible factor 2-alpha (HIF-2-alpha), (HIF2-alpha)
Member of PAS protein 2
PAS domain-containing protein 2
Gene name: EPAS1, BHLHE73, HIF2A, MOP2, PASD2
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung.

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Ref : 00024788

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Product Code pab50074
Immunogen Synthetic peptide derived from the C-term domain of mouse/human HIF-2 alpha protein.
Target Specificity Reacts with mouse, rat, fish and human HIF-2 alpha. Does not cross-react with HIF-1 alpha.
Name Hypoxia-inducible factor 2-alpha
Uniprot ID Q99814
Gene ID 2034
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Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human, Mouse, Rat, Fish
Labelling None
Appearance Liquid
Conc. / Activity 1.0 mg/ml
Form Antigen affinity purified
Constituents PBS
Preservatives NaN3 0.05%
Storage Store at 4°C. Do not freeze.
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation, Chromatin IP, Gel shift
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only:
WB (1.0 ug/ml - 2.0 ug/ml)
ChIP (1:10-1:500)
ELISA (1:100-1:2000)
ICC/IF (1:100)
IHC (1:100)
IP (5ug/1mg lysate)
Research areas Cancer,Hypoxia,Nuclear signaling,Signal transduction
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ICC/IF - HIF-2 alpha antibody<br/>(pab50074)<br/>Anti- HIF-2 alpha antibody ICC/IF staining (Green) of RCC4 cells. Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively. Antibody dilution 1:200

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