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HIF-1 alpha antibody

Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha)
ARNT-interacting protein
Basic-helix-loop-helix-PAS protein MOP1
Class E basic helix-loop-helix protein 78 (bHLHe78)
Member of PAS protein 1
PAS domain-containing protein 8
Gene name: HIF1A, BHLHE78, MOP1, PASD8
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

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Product Code pab50008
Immunogen Fusion protein containing amino acids 432-528 of human HIF-1 alpha.
Target Specificity Reacts with HIF-1 alpha from human, monkey, mouse, rat and Xenopus. Mouse results are mixed.
Name Hypoxia-inducible factor 1-alpha
Uniprot ID Q16665
Gene ID 3091
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Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human, Mouse, Rat, Monkey, Xenopus
Labelling None
Appearance Liquid
Conc. / Activity 1 mg/ml
Form Antigen affinity purified
Constituents Tris-glycine, 150 mM NaCl
Preservatives NaN3 0.05%
Storage Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Applications Western Blot, Immunohistochemistry, IHC - Paraffin sections, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin IP, Gel shift
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ChIP 25 ul for 15 million cells,
GS 1:1-1:100,
ICC/IF 1:10-1:2000,
IHC 1:10-1:2000,
IHC-P 1:100,
IP 1:1000,
WB 1:500-1:1000
Research areas Angiogenesis,Cancer,Developmental biology,Hypoxia,Nuclear signaling
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IHC: HIF-1 alpha antibody <br/>(pab50008)<br/>Anti-HIF-1 alpha antibody IHC staining of human kidney, renal tubular epithelium.

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