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Hypoxia inducible factor-1 alpha antibody (H1alpha67)

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, HIF1α
ARNT-interacting protein
Basic-helix-loop-helix-PAS protein MOP1
Class E basic helix-loop-helix protein 78, bHLHe78
Member of PAS protein 1
PAS domain-containing protein 8
Gene name: HIF1A, BHLHE78, MOP1, PASD8
HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis.This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 alpha is involved in B-1 B cell homeostasis, Notch signaling pathway and many other biological processes.

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Ref : 00024756

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Product Code mab50046
Clone H1alpha67
Immunogen Fusion protein containing amino acids 432-528 of human HIF-1alpha
Target Specificity Reacts with human HIF-1 alpha. Cross reacts with proteins from monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.
Name Hypoxia-inducible factor 1-alpha
Uniprot ID Q16665
Gene ID 3091
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Product type Primary antibodies
Clonality Monoclonal antibody
Isotype IgG2b
Produced in Mouse
Species Human, Mouse, Rat, Rabbit, Monkey, Sheep
Labelling None
Appearance Liquid
Conc. / Activity 1 mg/ml
Form Purified (protein A)
Constituents Tris-glycine, 150 mM NaCl
Preservatives NaN3 0.05%
Storage Store at 4°C. Do not freeze.
Applications Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation, Chromatin IP, Gel shift
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ChIP (1:10 - 1:500)
GS (1:1 - 1:100)
ICC/IF (1:50)
IHC (1:20 - 1:50)
IP (1:10 - 1:500)
WB: (1:500)
Research areas Angiogenesis,Cancer,Developmental biology,Hypoxia,Nuclear signaling
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IHC -  HIF-1 alpha antibody (H1alpha67)<br/>(mab50046)<br/>Anti-HIF-1 alpha antibody IHC staining of human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staini

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