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Histone H4 citrulline R3 antibody

HIST1H4
H4R3ci
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.

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Ref : 00014638

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Product Code pab0570
Immunogen Synthetic peptide derived from Nter region of Human Histone H4, containing Citrulline at position 3
Target Specificity Reacts with human Histone H4 Citrulline at the position 3 (10 kDa). No cross reaction with free H4 nor with other citrullinated proteins. May cross reacts with other species due to sequence homology.
Name Histone H4 citrulline R3
Print datasheet
Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human
Labelling None
Appearance Lyophilized powder
Form Antiserum
Preservatives None
Recommendations Must be reconstituted in distilled water.
Storage Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Antibody / Antigen pep0570
Applications Western Blot, Immunohistochemistry, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA (1:5000 - 1:10000)
IHC (1:100 - 1:1000)
WB (1:100 - 1:1000)
Research areas Chromatin,Post translational modifications
Print datasheet

ELISA - Histone H4 citrulline R3 antibody<br/>(pab0570)<br/>Control of the specificity of pab0570 using H4Cit3 peptide, H4-Agr3 or H3Cit2, 8, 17 peptide

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