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Histone H3 citrulline R2 + R8 + R17 antibody (7C10)

Histone H3.3C
Gene name: H3F3C
Histone H3 is citrullinated by PADI4 this modification induce a repression of transcription and impairs the methylation.

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Price From: €164.00
Ref : 00012694

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Product Code mab0072-P
Clone 7C10
Immunogen Synthetic peptide derived from Nter domain of human Histone H3 containing Citrulline at position 2-8-17
Target Specificity Reacts with human Histone H3 Citrulline at the 3 positions: 2, 8 and 17. No cross reaction with mono or bis H3-Cit.
Not tested in other species. May cross reacts with other species due to sequence homology.
Name Histone H3 citrulline R2 + R8 + R17
Uniprot ID Q6NXT2
Print datasheet
Product type Primary antibodies
Clonality Monoclonal antibody
Isotype IgG1 / Kappa
Produced in Mouse
Species Human
Labelling None
Appearance Lyophilized powder
Conc. / Activity 1 mg/ml
Form Purified (protein A)
Constituents Tris 0,1M, glycine 0,1M, sucrose 2%
Preservatives None
Recommendations Must be reconstituted in distilled water.
Storage Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Applications Immunofluorescence, Immunocytochemistry, Flow Cytometry, ELISA
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA 1:20 000 - 1:100 000
ICC/ IF 1:100 - 1:500
Research areas Epigenetic,Post translational modifications
Print datasheet
* Pircher J et al. Nat Commun. 2018 Apr 18,9(1):1523. (IF)

WB - Histone H3.3C antibody<br/>(mab0072-P)<br/>Anti-Histone H3.3C antibody WB staining of human Histone H3/Citrulunie 2-8-17. Antibody mab0072-P dilution 1 mg/ml

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