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Histone H3 citrulline R2 + R8 + R17 antibody

Histone H3.3C
Gene name: H3F3C
This core component of the nucleosome can be citrullinated on arginines 2, 8 and 17 by PADI4. These modifications blocks methylation and so represses the transcription

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Price From: €209.00
Ref : 00013175

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Product Code pab0208
Immunogen Synthetic peptide derived from Nter domain of human Histone H3 containing Citrulline at position 2-8-17
Target Specificity Reacts with human Histone H3 Citrulline at the 3 positions: 2, 8 and 17. Reacts with mono or di H3-Cit. Not tested in other species. May cross reacts with other species due to sequence homology.
Name Histone H3 citrulline R2 + R8 + R17
Uniprot ID Q6NXT2
Gene ID 440093
Print datasheet
Product type Primary antibodies
Clonality Polyclonal antibody
Produced in Rabbit
Species Human
Labelling None
Appearance Lyophilized powder
Form Antiserum
Preservatives None
Recommendations Must be reconstituted in distilled water.
Storage Lyophilized powder stable for a minimum of 2 years at -20°C.
Store reconstituted antibodies at +4°C.
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Antibody / Antigen pep0208
Applications Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, ELISA, Chromatin IP
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC : 1/2 000 to 1/20 000
- WB : 1/5 000 to 1/50 000
Research areas Epigenetic,Post translational modifications
Print datasheet
Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol. 2007 Apr 6;367(4):1118-29.

WB - Histone H3 citrulline R2 + R8 + R17 antibody<br/>(pab0208)<br/>Anti-H3 cit antibody WB staining of citrullinated histone H3. Antibody dilution 1:10000.

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