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His-Tag antibody (HIS.H8 / EH158)

poly-histidine, polyhistidine
Histidine tagged proteins
6 His epitope tag, HHHHHH epitope tag,
Hexa His tag, 6X His tag
His-Tag antibody (HIS.H8 / EH158)

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Price From: €123.00
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Ref : 00012927

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Product Code mab90001-P
Clone HIS.H8 / EH158
Immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH
Target Specificity Reacts with the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Name poly-histidine
Print datasheet
Product type Primary antibodies, Tags
Clonality Monoclonal antibody
Isotype IgG2b
Produced in Mouse
Labelling None
Appearance Liquid
Conc. / Activity 1 mg/mL
Form Purified
Constituents PBS 10mM, (pH 7.2)
Preservatives NaN3 0.05%
Storage Short term storage at +4°C (add 0.1% NaN3).
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Applications Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
WB 1:1000 - 1:3000
ICC 1:500 - 1:2000
Print datasheet
* Ossareh-Nazari B et al. Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy. J Cell Biol. 2014 Mar 17,204(6):909-17.

ICC - His-Tag antibody (HIS.H8 / EH158)<br/>(mab90001-P)<br/>Anti-His Tag antibody ICC staining of His-tag fusion protein in 293 cells.<br/>Left hand panel: untransfected control<br/>Right hand panel: transfected.<br/>Counterstained with DAPI (blue).

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