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Chemiluminescent reagent kit Covalight

The Covalight® chemiluminescent reagent for detecting proteins is extremely sensitive and has a high signal-to-noise ratio. This new non isotopic Covalight® system is a high performance chemiluminescent substrate for peroxidase. It enables less than 10 picograms of protein to be detected within 30 seconds to 1 minute on exposure to X-ray film.

If you want to order offline

orders@covalab.com - tel: +33 (0) 437 654 230 - fax: +33 (0) 437 289 416

Price From: €115.00
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Ref : 00028995

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Product Code opr0009
Constituents Reagent Cov A
Reagent Cov B
Print datasheet
Principle Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxidase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on spontaneous change to a stable form emits light. A dramatic increase in the light output can be achieved by the presence of enhancers.
Applications Western Blot, Dot Blot
Recommendations Chemiluminescence is defined as the light emitted by a chemical reaction. Horse radish peroxydase (HRP), in the presence of hydrogen peroxide, catalyses the oxidation of a cyclic diacyl hydrazide, such as luminol, to an excited reaction product which on s
Storage Stored at +4°C
Product type Reagents
Preparation method To be used extemporaneously or can be kept at +4°C for few hours : Add 1 drop of oxidant Cov B to 1 mL of substrat Cov A (125 µL of reagent covers 1 cm2 membrane).
Detection : Immerse the membrane in Covalight reagent from 1 to 10 minutes. Shake of excess reagent and cover the membrane with a plastic sheet before to exposure.
Print datasheet
* Chapuy-Regaud S et al. Biochimie. 2017 May 5.
* Brosse A et al. Nucl. Acids Res. 2016 Jul 20.
* Bloyet LM et al. J Virol. 2016 May 11.
* Fischer K. TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Ph.D. Thesis 2015.
* Julien M et al. Biochim Biophys Acta. 2015 Jun 14. pii: S0167-4889(15)00193-7.
* Romanets-Korbut O et al. Cell Mol Immunol. 2015 Jun 15.
* Couderc C. et al. Mol Cell Endocrinol. 2014 Sep 16. pii:S0303-7207(14)00284-6.
* Schnorr S et al. Pain. 2014 Jun:155(6):1079-90.
* Brot S, et al. Eur J Neurosci. 2014 Jul 12.
* Yakoub S et al. J Cancer Sci Ther 2014, 6.4
* Bollard J et al. Neuroendocrinology. 2013 Jan 23.
* Perez Alea M et al. Amino Acids. 2013 Jan:44(1):245-50

Chemiluminescent reagent kit Covalight

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