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Nε-(ϒ-L-glutamyl)-L-lysine isopeptide antibody (81D4)
isopeptide bond
As low as
$306.10
In stock
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00012721
Isopeptide bond are formed on lateral chains of proteins. Nε-(ϒ-L-glutamyl)-L-lysine isopeptide is generated after action of transglutaminase enzyme.
| ID Covalab | mab0012-P |
|---|---|
| Product type | Primary antibodies |
| Clonality | Monoclonal antibody |
| Clone | 81D4 |
| Isotype | IgM / Kappa |
| Raised in | Mouse |
| Immunogen | Nε-(ϒ-L-glutamyl)-L-lysine isopeptide |
| Activity cross reaction | Reacts with Ne (g-L-glutamyl)-L-lysine. Cross reacts in ELISA with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine. No cross reaction with : - protein bound Ne acetyl lysine - protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine - protein bound N1 mono (gPoly L-glutamyl) Spermidine - protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties. Ability to fix complement : unknown |
| Species | All Sp. |
| Labelling | None |
| Form | Purified |
| Preservative | None |
| Storage instructions | Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt. |
| Target | Nε-(ϒ-L-glutamyl)-L-lysine isopeptide |
| Applications | ELISA, IF, IHC |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : IHC (1:50 - 1:800) |
| Publication | * Serafini-Fracassini D et al. Plant Physiol Biochem. 2021 Jul 23:167:11-21 * Anal Biochem. 2020 Jan 7:113578 * Armstrong DMF et al. Acta Cir Bras. 2018 Nov,33(11):991-999. * Scarnato et al. LWT - Food Science and Technology 2017 Aug (81)101-110. * Zhang J et al. Mol Neurobiol. 2016 Oct;53(8):5066-78. * de Jager M et al. J Neurochem. J Neurochem. 2015 Sep,134(6):1116-28 * Steppan J et al. J Am Heart Assoc. 2014 Apr 10,3(2):e000599. (IHC, Rat) * Shrestha B et al. Biomed Res Int. 2014,2014:651608. (IHC, Rat) * Nadalutti CA et al. Cell Mol Life Sci. 2014 Apr,71(7):1315-26. * de Jager M. Neurobiol Aging. 2013 Apr,34(4):1159-69 * Shaik KS et al. Eur J Cell Biol. 2012 Mar,91(3):204-15. * Elli L et al. Lab Invest. 2011 Mar:91(3):452-61. (IHC, Rat) * Wang Z et al. PLoS Pathog. 2010 Feb 12,6(2):e1000763. (IHC, D. mel) * Thomas V et al. J Immunol Methods. 2004 Sep,292(1-2):83-95. * Johnson GV, LeShoure R Jr. J Neurosci Methods. 2004 Apr 30,134(2):151-8. * Kaartinen MT et al. J Bone Miner Res. 2002 Dec,17(12):2161-73. |
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