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Beta-actin antibody (BA3R)

Actin, cytoplasmic 1
Gene name: ACTB
Beta-actin is commonly used as loading control due to its ubiquitous expression in cells.

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Price From: €128.00
Ref : 00012917

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Product Code mab90010-P
Clone BA3R
Immunogen beta-Actin N-terminal peptide-KLH conjugates
Target Specificity Reacts with 42kDa native and denatured forms of beta-actin from human, mouse, rat, rabbit, and chicken.
Name Beta-actin
Uniprot ID P60709
Gene ID 60
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Product type Primary antibodies, Loading controls
Clonality Monoclonal antibody
Isotype IgG2b
Produced in Mouse
Species Human, Mouse, Rat, Rabbit, Chicken
Labelling None
Appearance Liquid
Conc. / Activity 1mg/ml
Form Purified
Constituents PBS 10mM, (pH 7.2)
Preservatives NaN3 0.05%
Storage Short term storage at +4°C (add 0.1% NaN3).
For extended periods store in aliquots at -20°C.
Antibodies are guaranteed for 6 month from date of receipt.
Applications Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation
Working dilutions Optimal dilution should be determined by the end user.
The following are guidelines only :
WB (1:1000 - 1:5000)
FC (0.1 - 1µg for 106^ cells)
ICC/IF (10 µg/ml)
IP (5 µg/ml)
Research areas Cell metabolism
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* Deygas M et al. Nat Commun. 2018 Oct 31;9(1):4545. (WB, Hu)

WB - Beta-actin antibody (BA3R)<br/>(mab90010-P)<br/>Anti-beta-actin antibody WB staining of cell lysates from (1) human, (2) mouse, (3) rat, (4) rabbit.

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